Equipment Model

Carl Zeiss Axio Scope.A1

Description

The Carl Zeiss Axio Scope.A1 is a modular, high-performance research microscope designed for brightfield, phase contrast, fluorescence, and other advanced contrast techniques. It is used for analyzing structural and functional details of living cells and tissues, and is especially well-suited for applications in cell biology, pathology, and material science.

Lab

CIF Room No: 3155

Institute

Central Analytical Laboratory

Location

CIF Room No: 3155, FD-III, Pilani Campus Vidya Vihar, Pilani, Rajasthan 333031, India

• Applications

  • Cell Biology: Detailed visualization of cellular structures, organelles, and dynamics in live or fixed cells.

  • Immunohistochemistry (IHC): Detection of specific antigens in tissues using labeled antibodies.

  • Immunofluorescence: Visualization of proteins and biomolecules via fluorescence-tagged antibodies.

  • Histopathology: Examination of tissue morphology for disease diagnosis and research.

  • Micro-manipulation: Precision handling and analysis of cells for genetic and cellular studies.

  • Developmental Biology: Observation of embryonic development and cell differentiation.

• Key Features

  • Modular design allowing configuration for transmitted light, fluorescence, DIC, and more.

  • High-resolution optics with Apochromatic objectives for precise imaging.

  • Ergonomic layout and smooth stage movement for extended use and comfort.

  • LED or halogen illumination with adjustable contrast techniques.

  • Compatible with digital imaging systems and software integration for image capture and analysis.

• Limitations

  • Requires proper staining and sample preparation for high-contrast visualization.

  • Fluorescence imaging may require light-sensitive handling and specific filters.

  • Limited to surface or thin-section analysis unless equipped with confocal or sectioning systems.

  • Maintenance of optics and alignment needed for consistent high-quality imaging.

  • Not suitable for ultra-structural imaging—requires electron microscopy for that level of detail.