Equipment Model

Carl Zeiss LSM 880

Description

The Carl Zeiss LSM 880 Fully Spectral Confocal Laser Microscope enables high-resolution imaging of biological and material samples by capturing multiple two-dimensional optical sections at various depths. These images can be reconstructed into detailed 3D visualizations, making it ideal for advanced fluorescence imaging and live cell studies across multiple scientific disciplines.

Lab

CIF Room No: 3182

Institute

Sophisticated Instrumentation Facility

Location

CIF Room No: 3182, FD-III, Pilani Campus Vidya Vihar, Pilani, Rajasthan 333031, India

• Applications

  • Cell biology: Imaging of live and fixed cells with high spatial resolution.

  • Developmental biology: Tracking cellular dynamics over time.

  • Genetics: Visualization of gene expression patterns using fluorescence markers.

  • Microbiology: Study of biofilms, microbes, and intracellular localization.

  • Pharmacy: Drug localization, distribution, and cellular uptake analysis.

  • Nanotechnology: High-resolution imaging of nanomaterials and nanoparticles in biological systems.

• Key Features

  • Fully spectral detection system allowing flexible fluorophore separation.

  • Z-stack imaging for 3D reconstruction of specimens.

  • Live-cell imaging capability with minimal photobleaching.

  • High sensitivity and resolution for fluorescence applications.

  • Multiple laser lines for simultaneous multi-channel imaging.

• Limitations

  • Requires fluorescent labeling for visualization.

  • Photobleaching and phototoxicity may occur during prolonged imaging.

  • Sample preparation can be complex and time-consuming.

  • Limited penetration depth for thick tissue imaging.

  • High cost of operation and maintenance.