Equipment Model

Carl Zeiss Axio Observer 7

Description

The Carl Zeiss Axio Observer 7 is an advanced inverted time-lapse microscope designed for live-cell imaging and long-term observation of biological samples. It offers high stability, precision, and imaging flexibility, enabling researchers to capture dynamic cellular processes over extended periods. The Axio Observer 7 integrates advanced optics, automated controls, and environmental regulation systems for consistent, high-quality imaging in cell biology, developmental biology, and medical research applications.

Lab

CIF Room No: 3182

Institute

Sophisticated Instrumentation Facility

Location

CIF Room No: 3182, FD-III, Pilani Campus Vidya Vihar, Pilani, Rajasthan 333031, India

• Applications

  • Live-cell imaging and long-term time-lapse studies

  • Observation of cell growth, migration, and division processes

  • Analysis of cellular responses to stimuli, drugs, and environmental changes

  • Fluorescence and phase-contrast imaging of biological samples

  • Quantitative imaging for cell tracking and morphometric analysis

  • Research in cell biology, neuroscience, developmental biology, and pharmacology

• Key Features

  • Inverted microscope design optimized for live-cell and tissue culture imaging

  • High-resolution fluorescence, DIC, and phase-contrast imaging modes

  • Fully motorized stage and focus control for automated time-lapse acquisition

  • Environmental chamber for temperature, CO₂, and humidity control during long-term imaging

  • Compatible with a wide range of objectives, cameras, and imaging modules

  • Integration with ZEN imaging software for automation, image analysis, and data management

• Limitations

  • Requires careful environmental control to maintain cell viability during long experiments

  • Complex setup and calibration process for advanced imaging modes

  • High cost of hardware, maintenance, and compatible imaging accessories

  • Image quality can be affected by photobleaching or phototoxicity during prolonged exposure

  • Requires trained personnel for operation and image analysis