Equipment Model

Thermo Fisher Scientific Ultimate 3000

Description

The Thermo Fisher Scientific Ultimate 3000 HPLC system is a modular, high-performance liquid chromatography platform designed for the separation, identification, and quantification of compounds in complex mixtures. It supports a wide range of analytical methods including reversed-phase, ion-exchange, size-exclusion, and normal-phase chromatography. Known for its reliability, resolution, and automation, the Ultimate 3000 is ideal for both routine and high-throughput analytical workflows.

Lab

CIF Room No: 3154

Institute

Central Analytical Laboratory

Location

CIF Room No: 3154, Birla Institute of Technology & Science, Vidya Vihar, Pilani, Rajasthan 333031, India

• Applications

  • Pharmaceutical Industry: Drug formulation analysis, purity testing, stability studies, and pharmacokinetics.

  • Environmental Analysis: Detection and quantification of pollutants, pesticides, and other organic contaminants.

  • Food & Beverage: Analysis of additives, preservatives, vitamins, and nutritional compounds.

  • Biotechnology: Protein, peptide, and nucleotide separation and analysis.

  • Clinical Research: Quantification of biomarkers, metabolites, and therapeutic compounds.

  • Chemical Industry: Quality control and compositional analysis of industrial chemicals and specialty compounds.

• Key Features

  • Modular design supporting gradient, isocratic, and UHPLC configurations.

  • High-resolution UV/Vis, diode array, and fluorescence detectors for enhanced sensitivity.

  • Precise solvent delivery system with micro-, analytical-, and preparative-scale capabilities.

  • Automated sample loading and temperature-controlled column compartments.

  • Integrated Chromeleon software for full control, method development, and compliance with regulatory standards (e.g., 21 CFR Part 11).

• Limitations

  • Not suitable for highly volatile compounds—requires GC or LC-MS for such analyses.

  • Method development may be time-intensive depending on sample complexity.

  • Requires regular maintenance of pump seals, detectors, and column hardware.

  • Initial setup and calibration can be complex for new users.

  • Cost of consumables (columns, solvents, filters) can be high over time.